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Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

DOI:
10.3791/58923-v

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07:49 min

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January 22, 2019

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, , , , , ,
1Centre for Molecular Medicine Norway, Faculty of Medicine,University of Oslo, 2Department of Chemistry, Faculty of Mathematics and Natural Sciences,University of Oslo, 3Department of Chemistry and Chemical Engineering,Chalmers University of Technology

Capitoli

  • 00:04Titolo
  • 00:54Preparation of Phospholipid Vesicle Suspension
  • 02:59Transformation of Molecular Phospholipid Films to Tubular Networks
  • 05:34Results: Transformation of Lipid Deposits to ER-Like Tubular Networks
  • 07:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

Solid-supported, protein-free, double phospholipid bilayer membranes (DLBM) can be transformed into complex and dynamic lipid nanotube networks and can serve as 2D bottom-up models of the endoplasmic reticulum.

Tags

Keywords: Lipid NanotubeEndoplasmic ReticulumBottom-up ModelProtein-free MembranePhospholipidsVesicle FormationMultilamellar VesiclesGiant Unilamellar VesiclesRotary EvaporationRehydrationSonication

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