Solid-supported, protein-free, double phospholipid bilayer membranes (DLBM) can be transformed into complex and dynamic lipid nanotube networks and can serve as 2D bottom-up models of the endoplasmic reticulum.
Köksal, E. S., Belletati, P. F., Reint, G., Olsson, R., Leitl, K. D., Kantarci, I., Gözen, I. Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum. J. Vis. Exp. (143), e58923, doi:10.3791/58923 (2019).