È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
A New Portable In Vitro Exposure Cassette for Aerosol Sampling
7,011 Views
•
07:01 min
•
February 22, 2019
DOI:
Summary
Automatically generated
Here, we present a protocol to perform portable cellular aerosol exposures and measure cellular response. The method uses cells, grown at the air-liquid interface, mimicking in vivo physiology. Cellular response to copper nanoparticle aerosols was observed as oxidative stress through reactive oxygen species generation and cytotoxicity as lactate dehydrogenase release.
Read Article
Cite this Article
Secondo, L. E., Wygal, N. J., Lewinski, N. A. A New Portable In Vitro Exposure Cassette for Aerosol Sampling. J. Vis. Exp. (144), e58916, doi:10.3791/58916 (2019).
Copy