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A New Portable In Vitro Exposure Cassette for Aerosol Sampling
Kapitel
- 00:04Titel
- 00:39Generation of Dry Aerosol
- 02:21Portable In Vitro Exposure Cassette (PIVEC) Assembly
- 03:45Aerosol Exposure of Cells
- 04:52Results: Deposition Efficiency and Expedited Post-exposure Analysis
- 06:00Conclusion
Here, we present a protocol to perform portable cellular aerosol exposures and measure cellular response. The method uses cells, grown at the air-liquid interface, mimicking in vivo physiology. Cellular response to copper nanoparticle aerosols was observed as oxidative stress through reactive oxygen species generation and cytotoxicity as lactate dehydrogenase release.
