/

/

生理活性类囊体的分离及其在能量依赖性蛋白转运检测中的应用

JoVE Journal
Biochimica

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biochimica

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Please note that all translations are automatically generated. Click here for the English version.

生理活性类囊体的分离及其在能量依赖性蛋白转运检测中的应用

10,051 Views

•

12:25 min

•

September 28, 2018

DOI:

10.3791/58393-v

DOI:

10.3791/58393-v

•

12:25 min

September 28, 2018

•

1 Views

Anthony Asher¹, Iniyan Ganesan¹, Laura Klasek¹, Steven M. Theg¹

¹Department of Plant Biology,University of California – Davis

Summary

本文提出了用于叶绿体双精氨酸易位 (cpTat)、分泌 (cpSec1) 和信号识别粒子 (cpSRP) 通路的生理活性类囊体和蛋白质转运测定的高产量隔离的协议。

Explore More Videos

Thylakoids Protein Transport Chloroplast Membranes Protein Targeting Percoll Gradient Chloroplast Isolation Pea Shoots Homogenization Centrifugation Chlorophyll Concentration

Read Article