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双光子活体显微镜感病毒感染时气管黏膜的影像细胞相互作用
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy
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双光子活体显微镜感病毒感染时气管黏膜的影像细胞相互作用

DOI:
10.3791/58355-v

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08:01 min

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August 17, 2018

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, , , ,
1Faculty of Biomedical Sciences, Institute for Research in Biomedicine,Università della Svizzera italiana (USI), 2Graduate School of Cellular and Molecular Sciences, Faculty of Medicine,University of Bern, 3Institute of Computational Science,Università della Svizzera italiana (USI)

Capitoli

  • 00:04Titolo
  • 00:57Intranasal Influenza Infection and Neutrophil Isolation and Injection
  • 03:21Surgical Preparation
  • 05:04In Vivo Time Lapse Imaging
  • 06:01Results: Representative Intravital 4D Imaging and Analyses
  • 07:28Conclusion

Summary

Traduzione automatica

Traduzione automatica

在本研究中, 我们提出了一个协议, 执行双光子活体成像和细胞相互作用分析的小鼠气管粘膜感染后流感病毒。这个协议将是相关的研究人员研究免疫细胞动力学在呼吸道感染。

Tags

Two-photon Intravital MicroscopyImmune ResponsesAirborne PathogensNeutrophil InteractionsInfluenza InfectionCD11c-YFP MousePR8 InoculateBone MarrowPercoll GradientNeutrophil Isolation

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