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Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy
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JoVE 杂志 免疫与感染
Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

DOI:
10.3791/58355-v

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08:01 min

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August 17, 2018

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, , , ,
1Faculty of Biomedical Sciences, Institute for Research in Biomedicine,Università della Svizzera italiana (USI), 2Graduate School of Cellular and Molecular Sciences, Faculty of Medicine,University of Bern, 3Institute of Computational Science,Università della Svizzera italiana (USI)

Chapters

  • 00:04Title
  • 00:57Intranasal Influenza Infection and Neutrophil Isolation and Injection
  • 03:21Surgical Preparation
  • 05:04In Vivo Time Lapse Imaging
  • 06:01Results: Representative Intravital 4D Imaging and Analyses
  • 07:28Conclusion

Summary

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In this study, we present a protocol to perform two-photon intravital imaging and cell interaction analysis in the murine tracheal mucosa after infection with influenza virus. This protocol will be relevant for researchers studying immune cell dynamics during respiratory infections.

Tags

Two-photon Intravital MicroscopyImmune ResponsesAirborne PathogensNeutrophil InteractionsInfluenza InfectionCD11c-YFP MousePR8 InoculateBone MarrowPercoll GradientNeutrophil Isolation

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