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Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
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JoVE Journal Biyokimya
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

DOI:
10.3791/58283-v

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09:14 min

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August 20, 2018

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, ,
1Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences,North Carolina State University

Bölümler

  • 00:04Başlık
  • 00:32Modification of a Baculovirus Expression Vector with either the GP67 Signal Peptideor the Insect Hemolin Signal Peptidefor Plant Protein Secretion Expression
  • 02:24Production and Amplification of Baculovirus Constructs Harboring the Recombinant Protein Expression Cassette
  • 07:02Results: Analysis of Plant Proteins Secreted by Insect Cells
  • 07:57Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we present the protocols for utilizing the insect cell and baculovirus protein expression system to produce large quantities of plant secreted proteins for protein crystallization. A baculovirus expression vector has been modified with either GP67 or insect hemolin signal peptide for plant protein secretion expression in insect cells.

Etiketler

Baculovirus Expression VectorsSecreted Plant ProteinsInsect CellsProtein Structure DeterminationRecombinant ProteinsDNA CloningDNA SequencingBacterial TransformationBacmid DNASf9 Cells

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