Rivista
/
Biologia dello sviluppo
/
Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells
JoVE Journal
Biologia dello sviluppo
This content is Free Access.
JoVE Journal Biologia dello sviluppo
Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells

Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells

7,841 Views

•

05:43 min

•

July 27, 2018

DOI:

10.3791/57995-v

DOI:
10.3791/57995-v

•

05:43 min
July 27, 2018

•

3 Views
, , ,
1MRC Centre for Regenerative Medicine,University of Edinburgh

Summary

Automatically generated

This protocol describes a semi-automated approach to produce hepatocyte-like cells from human pluripotent stem cells in a 96 well plate format. This process is rapid and cost-effective, allowing the production of quality assured batches of hepatocyte-like cells for basic and applied human research.

Explore More Videos

Semi-automatedHepatocyte-like CellsPluripotent Stem CellsLaminin 521MTeSR1 MediumROCK Inhibitor Y27632Definitive Endoderm InductionKSR DMSO MediumHepatoblast SpecificationHepatoZYME Medium96-well FormatAutomated Liquid HandlingMass ProductionCell ConfluencyDifferentiation

Read Article

Cite this Article

Meseguer-Ripolles, J., Lucendo-Villarin, B., Wang, Y., Hay, D. C. Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells. J. Vis. Exp. (137), e57995, doi:10.3791/57995 (2018).

Download .ris file

Copy

Share Video

.

Copy