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Capitoli
- 00:04Titolo
- 00:36Plant Excavation, Soil Removal, and Bulk Soil Collection
- 01:46Root and Rhizosphere Collection
- 02:44Root Surface Sterilization
- 03:54Processing Rhizosphere Samples
- 05:02DNA Extraction and Soil Analysis Processing
- 06:33DNA Extraction in 96-well Format
- 07:45Rhizosphere Sample Loading
- 08:47Results: Visualization and Statistical Methods Highlight Differences in Microbial Community Composition and Diversity Between Sample Types and Grass Species
- 10:03Conclusion
Udgravning af planternes rødder fra feltet samt behandling af prøver i endosphere, rhizosfære og jord er beskrevet i detaljer, herunder DNA-ekstraktion og data analysemetoder. Dette papir er beregnet til at aktivere andre laboratorier til at anvende disse metoder til undersøgelse af jordbundens, endosphere og rhizosfære microbiomes.
