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o 型血管哺乳动物细胞产生均匀集料的轨道晃动培养
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates
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o 型血管哺乳动物细胞产生均匀集料的轨道晃动培养

8,892 Views

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05:40 min

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January 07, 2019

DOI:

10.3791/57922-v

DOI:
10.3791/57922-v

•

05:40 min
January 07, 2019

•

6 Views
, , ,
1Department of Chemical System Engineering,University of Tokyo, 2Department of Biotechnology,Osaka University, 3Biotech Business Unit,Fukoku Co. Ltd, 4Institute of Industrial Science,University of Tokyo

Summary

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在这里, 我们提出了一个使用 o 型容器的协议, 专门用于悬浮培养的细胞集料, 轨道晃动。在这个袋子里生长的 hek293 细胞形成比传统培养容器中生长的细胞更均匀的聚集物。

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Cite this Article

Horiguchi, I., Suzuki, I., Morimura, T., Sakai, Y. An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels to Produce Uniform Aggregates. J. Vis. Exp. (143), e57922, doi:10.3791/57922 (2019).

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