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线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

线虫线虫中乳酸和丙酮酸细胞的小尺度比色测定

DOI:

09:35 min

October 15, 2018

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Capitoli

  • 00:04Titolo
  • 00:37Caenorhabditis elegans Synchronized Culture
  • 03:21Extraction of Cellular Fraction from C. elegans
  • 05:35Lactate, Pyruvate and Protein Assays
  • 07:32Results: Efficiency of Lactate and Pyruvate Colorimetric Assays After Protein Precipitation of C. elegans
  • 08:26Conclusion

Summary

Traduzione automatica

本文介绍了一种改良的小尺度萃取和比色法测定乳酸和丙酮酸在线虫中的含量.使用商用试剂盒时, 其灵敏度和准确度的技术发展非常重要。在萃取中蛋白质沉淀是定量测定细胞内代谢产物的关键步骤。

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