Rivista
/
Genetica
/
En Ecdysone Receptor-baserede ental gen Switch til bevidst udtryk for transgen med robusthed, reversibilitet og ubetydelig Leakiness
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

En Ecdysone Receptor-baserede ental gen Switch til bevidst udtryk for transgen med robusthed, reversibilitet og ubetydelig Leakiness

6,353 Views

•

06:21 min

•

May 07, 2018

DOI:

10.3791/57494-v

DOI:
10.3791/57494-v

•

06:21 min
May 07, 2018

•

4 Views
, , , ,
1Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, 2Department of Pharmacology, College of Medicine,Chungnam National University, 3Department of Nursing,Gwangju Women’s University

Summary

Automatically generated

Vi præsenterer her, en protokol om en graduering af transgenet udtryk ved hjælp af pEUI(+) ental gen switch ved tebufenozide behandling.

Explore More Videos

Ecdysone ReceptorGene SwitchTransgene ExpressionTebufenozideHEK 293 CellsEGFPANKRD13ATransfectionImmunoblotting

Read Article

Cite this Article

Lee, S., Won, M., Hwang, R. H., Hur, G. M., Ro, H. An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness. J. Vis. Exp. (135), e57494, doi:10.3791/57494 (2018).

Download .ris file

Copy

Share Video

.

Copy