Journal
/
Genetik
/
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
JoVE Journal
Genetik
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Genetik
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

6,353 Views

•

06:21 min

•

May 07, 2018

DOI:

10.3791/57494-v

DOI:
10.3791/57494-v

•

06:21 min
May 07, 2018

•

4 Views
, , , ,
1Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, 2Department of Pharmacology, College of Medicine,Chungnam National University, 3Department of Nursing,Gwangju Women’s University

Summary

Automatically generated

Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment.

Explore More Videos

Ecdysone ReceptorGene SwitchTransgene ExpressionTebufenozideHEK 293 CellsEGFPANKRD13ATransfectionImmunoblotting

Read Article

Cite this Article

Lee, S., Won, M., Hwang, R. H., Hur, G. M., Ro, H. An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness. J. Vis. Exp. (135), e57494, doi:10.3791/57494 (2018).

Download .ris file

Copy

Share Video

.

Copy