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An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
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遗传学
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JoVE 杂志 遗传学
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

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06:21 min

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May 07, 2018

DOI:

10.3791/57494-v

DOI:
10.3791/57494-v

•

06:21 min
May 07, 2018

•

4 Views
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1Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, 2Department of Pharmacology, College of Medicine,Chungnam National University, 3Department of Nursing,Gwangju Women’s University

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Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment.

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Ecdysone ReceptorGene SwitchTransgene ExpressionTebufenozideHEK 293 CellsEGFPANKRD13ATransfectionImmunoblotting

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Lee, S., Won, M., Hwang, R. H., Hur, G. M., Ro, H. An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness. J. Vis. Exp. (135), e57494, doi:10.3791/57494 (2018).

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