Rivista
/
Genetica
/
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
JoVE Journal
Genetica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Genetica
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

DOI:
10.3791/57494-v

•

06:21 min

•

May 07, 2018

•

, , , ,
1Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, 2Department of Pharmacology, College of Medicine,Chungnam National University, 3Department of Nursing,Gwangju Women’s University

Capitoli

  • 00:05Titolo
  • 00:45Transient Transfection and Teb Treatment
  • 03:00Depletion of Teb
  • 04:01Results: Modulation of Transgene Expression of the pEUI() Singular Gene Switch using Teb
  • 05:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol for the modulation of transgene expression using pEUI(+) singular gene switch by tebufenozide treatment.

Tags

Ecdysone ReceptorGene SwitchTransgene ExpressionTebufenozideHEK 293 CellsEGFPANKRD13ATransfectionImmunoblotting

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Read Article