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增强基因组编辑与 Cas9 Ribonucleoprotein 在不同的细胞和有机体
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May 25, 2018
DOI:
1Department of Molecular Cell Biology,University of California, Berkeley, 2Howard Hughes Medical Institute,University of California, Berkeley, 3Innovative Genomics Institute,University of California, Berkeley, 4Biomedical Sciences Graduate Program,University of California, San Francisco, 5Department of Microbiology and Immunology,University of California, San Francisco, 6Diabetes Center,University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine,University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center,University of California, San Francisco, 10Department of Integrative Biology,University of California, Berkeley
Summary
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利用预装 Cas9 ribonucleoprotein 复合体 (RNP) 是精确、高效的基因组编辑的有力方法。在这里, 我们强调它的效用横跨广泛的细胞和有机体, 包括主要的人类细胞和经典和新兴的模型有机体。
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Cite this Article
Farboud, B., Jarvis, E., Roth, T. L., Shin, J., Corn, J. E., Marson, A., Meyer, B. J., Patel, N. H., Hochstrasser, M. L. Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms. J. Vis. Exp. (135), e57350, doi:10.3791/57350 (2018).
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