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Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
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Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
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February 15, 2018
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A detailed protocol to perform a titration ELISA is described. Moreover, a novel algorithm is presented to evaluate titration ELISAs and to obtain a dissociation constant of binding of a soluble ligand to a microtiter plate-immobilized receptor.
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Eble, J. A. Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction. J. Vis. Exp. (132), e57334, doi:10.3791/57334 (2018).
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