Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
19,203 Views
•
12:38 min
•
February 15, 2018
DOI:
Summary
Automatically generated
A detailed protocol to perform a titration ELISA is described. Moreover, a novel algorithm is presented to evaluate titration ELISAs and to obtain a dissociation constant of binding of a soluble ligand to a microtiter plate-immobilized receptor.
Read Article
Cite this Article
Eble, J. A. Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction. J. Vis. Exp. (132), e57334, doi:10.3791/57334 (2018).
Copy