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Replicatie van de besteld, Nonredundant bibliotheek van Pseudomonas aeruginosa stam PA14 Transposon insertiemutanten
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
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Replicatie van de besteld, Nonredundant bibliotheek van Pseudomonas aeruginosa stam PA14 Transposon insertiemutanten

7,916 Views

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11:35 min

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May 04, 2018

DOI:

10.3791/57298-v

DOI:
10.3791/57298-v

•

11:35 min
May 04, 2018

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2 Views
, , , , , , ,
1Department of Pediatrics, Mucosal Immunology and Biology Research Center,Massachusetts General Hospital, 2Department of Molecular Biology,Massachusetts General Hospital, 3Department of Genetics,Harvard Medical School, 4Department of Pediatrics,Harvard Medical School

Summary

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Pseudomonas aeruginosa infectie veroorzaakt significante morbiditeit in kwetsbare hosts. De nonredundant transposon invoeging mutant bibliotheek van P. aeruginosa stam PA14, uitgeroepen tot PA14NR ingesteld, vergemakkelijkt de analyse van gene functionaliteit in tal van processen. Hier gepresenteerd is een protocol voor het genereren van hoge kwaliteit kopieën van de PA14NR ingesteld mutant bibliotheek.

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Cite this Article

Drenkard, E., Hibbler, R. M., Gutu, D. A., Eaton, A. D., Silverio, A. L., Ausubel, F. M., Hurley, B. P., Yonker, L. M. Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants. J. Vis. Exp. (135), e57298, doi:10.3791/57298 (2018).

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