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Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
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Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

DOI:
10.3791/57240-v

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09:23 min

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June 29, 2018

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, , , , , ,
1Department of Biological Sciences,University of South Carolina, 2Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences, 3Department of Instructions and Teacher Education,University of South Carolina

Capitoli

  • 00:04Titolo
  • 00:49Isolation of Genomic DNA from Plant Tissue
  • 05:04Perform PCR on Genomic DNA Samplesand Electrophoresis of the Amplified DNA II. Isolation of Genomic DNA from Plant Tissueon an Agarose Gel
  • 07:34Results: PCR Amplification of Genomic DNA to Identify the Citrus Greening Bacterium
  • 08:52Conclusion

Summary

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Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp.

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Keywords: Citrus GreeningCandidatus Liberibacter Spp.PCR DetectionPathogen IdentificationCitrus FarmingQuantitative AnalysisLaboratory ProcedureRNA RemovalDNA PrecipitationCentrifugation

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