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Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples
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环境科学
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JoVE 杂志 环境科学
Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

7,512 Views

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09:23 min

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June 29, 2018

DOI:

10.3791/57240-v

DOI:
10.3791/57240-v

•

09:23 min
June 29, 2018

•

2 Views
, , , , , ,
1Department of Biological Sciences,University of South Carolina, 2Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences, 3Department of Instructions and Teacher Education,University of South Carolina

Summary

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Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp.

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Cite this Article

Chen, H., Palmer, I. A., Chen, J., Chang, M., Thompson, S. L., Liu, F., Fu, Z. Q. Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples. J. Vis. Exp. (136), e57240, doi:10.3791/57240 (2018).

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