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清醒小鼠海马毛细血管的体内纤维耦合临床前共聚焦激光扫描内窥镜 （pCLE）
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice
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清醒小鼠海马毛细血管的体内纤维耦合临床前共聚焦激光扫描内窥镜 （pCLE）

DOI:
10.3791/57220-v

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09:08 min

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April 21, 2023

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, ,
1División de Neurociencias,Universidad Pablo de Olavide, 2Barrow Neurological Institute, 3Ophthalmology, Neurology, and Physiology/Pharmacology,SUNY Downstate Medical Center

Capitoli

  • 00:05Introduction
  • 01:18Stereotaxic Positioning
  • 03:27Head-Cap Installation
  • 04:33Imaging Window
  • 05:55In Vivo Fiber-Optic-Bundle-Coupled Pre-Clinical Confocal Laser-Scanning Endomicroscopy (pCLE)
  • 07:27Results: Representative Mural Cell Vasoconstriction and Recorded Brain Immunohistochemistry
  • 08:26Conclusion

Summary

Traduzione automatica

Traduzione automatica

虽然多光子成像仅在距组织表面的有限深度有效，但可以通过pCLE在任何深度实现3μm分辨率成像。在这里，我们提出了一种进行pCLE成像的方案，以测量发作期和野生型小鼠海马体中的微血管动力学。

Tags

Keywords: In VivoFiber-coupledPre-clinicalConfocalLaser-scanningEndomicroscopyPCLEHippocampalCapillariesAwakeMiceRestraint MethodBlood FlowVascular AbnormalitiesEpilepsyAge-related Macular DegenerationRetinaStereotaxic FrameSkullIncisionPeriosteumBregmaHippocampusImaging Window

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