Rivista
/
Ricerca sul cancro
/
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
JoVE Journal
Ricerca sul cancro
This content is Free Access.
JoVE Journal Ricerca sul cancro
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

DOI:
10.3791/57215-v

•

05:22 min

•

April 13, 2018

•

, ,
1Koch Institute for Integrative Cancer Research at MIT, Department of Biology,Massachusetts Institute of Technology

Capitoli

  • 00:04Titolo
  • 00:32Synchronization of RPE-1 hTERT Cells
  • 01:34Generation of Aneuploid Cells by Interference with the Activity of the Mitotic Kinase Mps1 and Removal of Aneuploid Cycling Cells to Enrich the ArCK Population
  • 03:36Results: In Vitro Isolation and Characterization of Aneuploid Cells with Complex Karyotypes (ArCK)
  • 04:35Conclusion

Summary

Traduzione automatica

Traduzione automatica

Aneuploidy leads to genome instability, which eventually produces cell cycle-arrested cells with complex karyotypes. This paper provides a simple and convenient method to isolate aneuploid cells with complex karyotypes that cease to divide.

Tags

Cell Cycle ArrestComplex KaryotypesAneuploid CellsCell SynchronizationRPE-1 HTERT CellsThymidineMps1 Inhibitor ReversineNocodazoleMitotic Shake-offArCK Cells

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

Decellularization of the Murine Cardiopulmonary Complex

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Read Article