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中枢神经元轴突静脉曲张形成和恢复的 Microbiomechanical 系统研究

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Neuroscienze

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JoVE Journal Neuroscienze

A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons

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中枢神经元轴突静脉曲张形成和恢复的 Microbiomechanical 系统研究

DOI:

10.3791/57202-v

•

09:58 min

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April 30, 2018

•

Dustin Servello, Yuanzheng Gu³, Chen Gu²

¹Molecular, Cellular, and Developmental Biology Program,The Ohio State University, ²Department of Biological Chemistry and Pharmacology,The Ohio State University, ³Biogen

Capitoli

00:05Titolo
00:55Coverslip Preparation
02:43Dissociation and Culture of Hippocampal Neurons
06:41Setting Up the Puffing Pipette Apparatus
08:27Results: Representative Images of Varicosity Formation Following Puffing
09:09Conclusion

Summary

Traduzione automatica

本协议描述了一种生理上相关的加压液方法, 用于快速可逆地诱导神经元中的静脉曲张。

Tags

Keywords: Microbiomechanical System Varicosity Formation Axon Recovery Central Neuron Brain Injury Neuronal Plasticity Drug Screening Traumatic Brain Injury Hippocampal Neuron Culture Protease Enzyme Plating Medium Subcellular Morphology Mechanical Stress

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