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从酿酒酵母中组蛋白修饰的染色质沉淀 (芯片)

JoVE Journal
Genetica

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JoVE Journal Genetica

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae

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从酿酒酵母中组蛋白修饰的染色质沉淀 (芯片)

12,438 Views

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11:06 min

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December 29, 2017

DOI:

10.3791/57080-v

DOI:

10.3791/57080-v

•

11:06 min

December 29, 2017

•

12 Views

Meagan Jezek¹, Alison Jacques¹, Deepika Jaiswal¹, Erin M. Green¹

¹Department of Biological Sciences,University of Maryland

Summary

在这里, 我们描述了一个协议的染色质沉淀的修饰组蛋白从萌芽酵母酿酒酵母。Immunoprecipitated DNA 随后用于定量 PCR 研究组蛋白修饰修饰的丰度和局部化。

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