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利用受刺激的排放损耗 (STED) 显微镜可视化肌动蛋白和微管骨架在 B 细胞免疫突触中的应用
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy
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利用受刺激的排放损耗 (STED) 显微镜可视化肌动蛋白和微管骨架在 B 细胞免疫突触中的应用

DOI:
10.3791/57028-v

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11:00 min

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April 09, 2018

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, ,
1Department of Microbiology and Immunology,University of British Columbia

Capitoli

  • 00:05Titolo
  • 01:18GFP-Fusion Protein Expression and Anti-IgG Antibody Glass Coverslip Coating
  • 03:14Cell Spreading, Fixing, and Immunostaining
  • 05:44Stimulated Emission Depletion Microscope Imaging
  • 08:02Results: Representative STED Imaging
  • 09:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们提出了一个使用 STED 显微术的协议, 同时图像肌动蛋白结构, 微管和微管加端结合蛋白的 b 细胞已扩散到盖玻片的抗体涂层的 b 细胞受体, 一个模型的初始阶段免疫突触形成。

Tags

ActinMicrotubuleCytoskeletonB-cellImmune SynapseSTED MicroscopyCytoskeleton OrganizationCytoskeleton ReorganizationImmune Cell ActivationFluorophoresA20 B-lymphoma CellsTransfectionGoat Anti-mouse Immunoglobulin G AntibodyCoverslips

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