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Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments
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Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments
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May 09, 2018
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Summary
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Imaging retinal tissue can provide single-cell information that cannot be gathered from traditional biochemical methods. This protocol describes preparation of retinal slices from zebrafish for confocal imaging. Fluorescent genetically encoded sensors or indicator dyes allow visualization of numerous biological processes in distinct retinal cell types.
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Giarmarco, M. M., Cleghorn, W. M., Hurley, J. B., Brockerhoff, S. E. Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments. J. Vis. Exp. (135), e56977, doi:10.3791/56977 (2018).
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