/
/
Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments
8,978 Views
•
11:01 min
•
May 09, 2018
DOI:
Summary
Automatically generated
Imaging retinal tissue can provide single-cell information that cannot be gathered from traditional biochemical methods. This protocol describes preparation of retinal slices from zebrafish for confocal imaging. Fluorescent genetically encoded sensors or indicator dyes allow visualization of numerous biological processes in distinct retinal cell types.
Read Article
Cite this Article
Giarmarco, M. M., Cleghorn, W. M., Hurley, J. B., Brockerhoff, S. E. Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments. J. Vis. Exp. (135), e56977, doi:10.3791/56977 (2018).
Copy