Rivista
/
Chimica
/
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
JoVE Journal
Chimica
This content is Free Access.
JoVE Journal Chimica
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials

4,036 Views

•

07:54 min

•

October 27, 2020

DOI:

10.3791/61760-v

DOI:
10.3791/61760-v

•

07:54 min
October 27, 2020

•

1 Views
, , , , , ,
1School of Geography Earth and Environmental Sciences,University of Birmingham, 2Biomedical Microscale Analytics,Leiden University, 3Institute of Medical Biochemistry,Medical University of Innsbruck, 4AB Sciex UK Ltd

Summary

Automatically generated

Here we present a protocol to characterize the complete biomolecular corona, proteins, and metabolites, acquired by nanomaterials from biofluids using a capillary electrophoresis – mass spectrometry approach.

Explore More Videos

Capillary ElectrophoresisMass SpectrometryCESI-MSProtein CoronaMetabolite CoronaNanomaterialsHuman PlasmaProtein CharacterizationMetabolite CharacterizationTrypsin DigestionC18 DesaltingSample Preparation

Read Article

Cite this Article

Chetwynd, A. J., Zhang, W., Faserl, K., Thorn, J. A., Lynch, I., Ramautar, R., Lindner, H. H. Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials. J. Vis. Exp. (164), e61760, doi:10.3791/61760 (2020).

Download .ris file

Copy

Share Video

.

Copy