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Metaboliska märkning och profilering av överföring RNAs använder Macroarrays
JoVE Journal
Genetica
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JoVE Journal Genetica
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
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Metaboliska märkning och profilering av överföring RNAs använder Macroarrays

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10:56 min

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January 16, 2018

DOI:

10.3791/56898-v

DOI:
10.3791/56898-v

•

10:56 min
January 16, 2018

•

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1Laboratory of tRNA Biology, Department of Biology,College of Charleston, 2Department of Biochemistry and Molecular Biology,MUSC, 3Department of Biology,College of Charleston

Tags

Metabolic LabelingTRNA ProfilingMacroarray AnalysisTRNA RegulationProtein SynthesisMycobacterium SmegmatisPhosphorus-32 LabelingRNA ExtractionChloroform Extraction

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Emetu, S., Troiano, M., Goldmintz, J., Tomberlin, J., Grelet, S., Howe, P. H., Korey, C., Geslain, R. Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays. J. Vis. Exp. (131), e56898, doi:10.3791/56898 (2018).

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