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Metaboliske mærkning og profilering af overførsel RNA'er ved hjælp af Macroarrays
JoVE Journal
Genetica
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JoVE Journal Genetica
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays

Metaboliske mærkning og profilering af overførsel RNA'er ved hjælp af Macroarrays

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10:56 min

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January 16, 2018

DOI:

10.3791/56898-v

DOI:
10.3791/56898-v

•

10:56 min
January 16, 2018

•

1 Views
, , , , , , ,
1Laboratory of tRNA Biology, Department of Biology,College of Charleston, 2Department of Biochemistry and Molecular Biology,MUSC, 3Department of Biology,College of Charleston

Summary

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Vi beskriver en oprindelige transfer RNA analyse platform kaldet SPOt (strømlinet Platform for observere tRNA). SPOt samtidig foranstaltninger cellulære niveauer af alle tRNAer i biologiske prøver, i kun tre trin, og i mindre end 24 timer.

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Cite this Article

Emetu, S., Troiano, M., Goldmintz, J., Tomberlin, J., Grelet, S., Howe, P. H., Korey, C., Geslain, R. Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays. J. Vis. Exp. (131), e56898, doi:10.3791/56898 (2018).

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