Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Generation and Culturing of Primary Human…
JoVE Journal
Developmental Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Developmental Biology
Generation and Culturing of Primary Human Keratinocytes from Adult Skin
 

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

Article DOI: 10.3791/56863-v • 10:42 min • December 22nd, 2017
December 22nd, 2017 •
1
1Department of Dermatology, Aarhus University Hospital

Capitoli

Riepilogo
Automatic Translation

The human skin acts as a first line of defense against the external environment. We present a method for isolating primary human keratinocytes from adult skin. These isolated keratinocytes are useful in numerous experimental setups, and are a highly suitable model for studying molecular mechanisms in cutaneous biology in vitro.

Tags

Generation Culturing Primary Human Keratinocytes Adult Skin Model Cutaneous Biology Epidermal Cell Biology Keratinocyte Differentiation Inflammatory Skin Diseases Isolation Men Women Age Procedure Annette Blak Rasmussen Technician Solution Trypsin Glucose DPBS RPMI 1640 FBS KSFM Supplements Gentamicin Pre-warm Skin Samples Volunteers Plastic Surgery
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter