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Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

DOI:
10.3791/56801-v

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10:06 min

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February 26, 2018

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, , ,
1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Capitoli

  • 00:04Titolo
  • 01:02Preparing the M. tuberculosis Inoculum
  • 03:13Inoculating Mice with Airborne M. tuberculosis
  • 05:33Reporter Enzyme Fluorescence Imaging and Analysis of Lung Homogenate
  • 08:05Results: Imaging
  • 09:32Conclusion

Summary

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We describe the optical imaging of mice infected with Mycobacterium tuberculosis (M. tuberculosis) using reporter enzyme fluorescence (REF). This protocol facilitates the sensitive and specific detection of M. tuberculosis in pre-clinical animal models for pathogenesis, therapeutics and vaccine research.

Tags

Reporter Enzyme FluorescenceMycobacterium TuberculosisPre-clinical Animal ModelsPathogenesisTherapeuticsVaccine ResearchVirulence MechanismsHost-pathogen InteractionsMOADCTW Medium7H11 PlatesCFUBiosafety HoodNebulizerInoculation Chamber

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