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Mycobacterium tuberculosis em camundongos com fluorescência de enzima do repórter de imagem
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

Mycobacterium tuberculosis em camundongos com fluorescência de enzima do repórter de imagem

7,092 Views

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10:06 min

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February 26, 2018

DOI:

10.3791/56801-v

DOI:
10.3791/56801-v

•

10:06 min
February 26, 2018

•

2 Views
, , ,
1Department of Microbial Pathogenesis and Immunology,Texas A&M Health Science Center, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases,National Institutes of Health

Summary

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Descrevemos a imagem óptica de camundongos infectados com Mycobacterium tuberculosis (M. tuberculosis) usando fluorescência de enzima repórter (REF). Esse protocolo proporciona uma detecção sensível e específica de M. tuberculosis em modelos animais pré-clínicos para investigação patogenia, terapêutica e vacina.

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Cite this Article

Sharan, R., Yang, H., Sule, P., Cirillo, J. D. Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence. J. Vis. Exp. (132), e56801, doi:10.3791/56801 (2018).

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