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一种简便、低成本的肌营养不良小鼠步行模型的测定方法
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A Simple and Low-cost Assay for Measuring Ambulation in Mouse Models of Muscular Dystrophy
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一种简便、低成本的肌营养不良小鼠步行模型的测定方法

DOI:
10.3791/56772-v

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05:54 min

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December 29, 2017

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1Department of Integrative Biology and Physiology,University of California, 2Center for Duchenne Muscular Dystrophy,University of California, 3Department of Neurology,David Geffen School of Medicine, University of California, 4Molecular Biology Institute,University of California

Capitoli

  • 00:05Titolo
  • 00:43Chamber Preparation, Muscle Activation, and Ambulation Recording
  • 01:50Video Analysis
  • 03:28Results: Analysis of mdx Ambulation and Representative Effects of Exercise
  • 05:16Conclusion

Summary

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该协议描述了一个灵活的, 低成本的测量鼠标移动在野外活动的测试系统。我们显示, 基于这个系统的6分钟的步行试验检测到在mdx小鼠中自愿运动的减少, 并准确地区分了这些动物的肌肉特异性抢救的改进。

Tags

Keywords: Muscular DystrophyMouse ModelAmbulationActivityGrip StrengthVideo TrackingNon-invasiveReproducible

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