Rivista
/
Immunologia e infezioni
/
Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

8,001 Views

•

12:57 min

•

November 16, 2017

DOI:

10.3791/56444-v

DOI:
10.3791/56444-v

•

12:57 min
November 16, 2017

•

2 Views
, ,
1Department of Food, Bioprocessing, and Nutrition Sciences,North Carolina State University, 2Department of Chemical and Biomolecular Engineering,North Carolina State University

Summary

Automatically generated

Routine detection methods utilizing viral genome amplification are limited by their inability to discriminate infectious from non-infectious particles. The purpose of this article is to provide detailed protocols for alternative methods to aid in discrimination of infectious norovirus particles using aptamer binding, dynamic light scattering, and transmission electron microscopy.

Explore More Videos

Viral CapsidStructural IntegrityIn Vitro MethodsNorovirusViral InactivationCapsid ProteinHeat TreatmentAptamerELISA

Read Article

Cite this Article

Moore, M. D., Mertens, B. S., Jaykus, L. Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity. J. Vis. Exp. (129), e56444, doi:10.3791/56444 (2017).

Download .ris file

Copy

Share Video

.

Copy