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Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates

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Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates

Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates

DOI:

10.3791/56330-v

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08:32 min

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October 03, 2017

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Leah C Acker^2,3, Erica N. Pino^4,5, Edward S. Boyden^6,7, Robert Desimone⁷

¹McGovern Institute,Massachusetts Institute of Technology, ²Harvard-MIT Division of Heath Sciences and Technology, ³Department of Anesthesiology,Duke University Medical Center, ⁴Department of Biology,Massachusetts Institute of Technology, ⁵Division of Pharmacoengineering and Molecular Pharmaceutics, UNC Eshelman School of Pharmacy,University of North Carolina at Chapel Hill, ⁶Media Lab and Department of Biological Engineering,Massachusetts Institute of Technology, ⁷Department of Brain and Cognitive Sciences,Massachusetts Institute of Technology

Capitoli

00:05Titolo
01:03Illuminator Fabrication
05:09In Vivo Illumination
07:07Results: Behavioral and Electrophysiological Effects of Optogenetic Inhibition with Large Volume Illumination
07:32Conclusion

Summary

Traduzione automatica

A protocol to build a tissue penetrating illuminator for delivering light over large volumes with minimal diameter is presented.

Tags

Keywords: Optogenetics Non-human Primates Neuronal Firing Translational Medicine Plastic Optical Fiber Heat Gun Fiber Thinning Constant Tension

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