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Multiciliated 셀 전체 마운트 형광 제자리에서 교 잡 및 면역 형광의 결합 된 프로토콜을 통해 Zebrafish에 시각화
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
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Multiciliated 셀 전체 마운트 형광 제자리에서 교 잡 및 면역 형광의 결합 된 프로토콜을 통해 Zebrafish에 시각화

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09:33 min

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November 18, 2017

DOI:

10.3791/56261-v

DOI:
10.3791/56261-v

•

09:33 min
November 18, 2017

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2 Views
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1Department of Biological Sciences,University of Notre Dame

Tags

Multiciliated CellsZebrafishWhole Mount Fluorescent In Situ HybridizationImmunofluorescenceBiologia dello sviluppoPronephrosCiliaRNA TranscriptsProtein LabelingAntibodiesFixationRehydrationProteinase KHybridization SolutionRNA Probe

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Marra, A. N., Ulrich, M., White, A., Springer, M., Wingert, R. A. Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence. J. Vis. Exp. (129), e56261, doi:10.3791/56261 (2017).

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