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Neuroscience

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JoVE Journal Neuroscience
Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish
 

Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

Article DOI: 10.3791/56249-v • 06:27 min • October 27th, 2017
October 27th, 2017 •
1,2,3, 1, 1,2,3, 1, 1,2
1Department of Ophthalmology, University Hospital of Bern, University of Bern, 2Department of Clinical Research, University of Bern, 3Graduate School for Cellular and Biomedical Sciences, University of Bern

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The zebrafish is a popular animal model to study mechanisms of retinal degeneration/regeneration in vertebrates. This protocol describes a method to induce localized injury disrupting the outer retina with minimal damage to the inner retina. Subsequently, we monitor in vivo the retinal morphology and the Müller glia response throughout retinal regeneration.

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Müller Glia Cell Activation Laser-induced Retinal Degeneration Regeneration Model Zebrafish Cellular Changes In Vivo Monitoring Focal Injury Retinal Regeneration Morphological Changes Kinetics Cell Types Involved Repair Mechanism Anesthetic Solution Tricaine Powder Stock Solution Working Solution Petri Dish Zebrafish Immobilization Silicone Pin Holder Laser Treatment
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