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AFM and Microrheology in the Zebrafish Embryo Yolk Cell
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
AFM and Microrheology in the Zebrafish Embryo Yolk Cell

AFM and Microrheology in the Zebrafish Embryo Yolk Cell

DOI:

09:47 min

November 29, 2017

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Capitoli

  • 00:05Titolo
  • 01:08Atomic Force Microscopy
  • 03:54Particle Tracking Microrheology
  • 07:02Results: Cortical Tension Measurements and Rheology of the Cortex and Yolk of the Zebrafish Embryo Yolk Cell During Epiboly
  • 08:46Conclusion

Summary

Traduzione automatica

The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.

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