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多光子时间推移成像实时可视化发展： 迁移神经嵴细胞在斑马鱼胚胎中的可视化
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
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多光子时间推移成像实时可视化发展： 迁移神经嵴细胞在斑马鱼胚胎中的可视化

DOI:
10.3791/56214-v

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10:13 min

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August 09, 2017

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1Department of Ophthalmology and Visual Sciences, Kellogg Eye Center,University of Michigan

Capitoli

  • 00:05Titolo
  • 00:48Embryo Collection for Time-lapse Imaging
  • 01:51Embryo Preparation
  • 02:52Preparation of 2% Low-melt Agarose and Mounting the Embryo
  • 04:34Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition
  • 07:26Post-acquisition Processing
  • 08:10Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos
  • 09:29Conclusion

Summary

Traduzione automatica

Traduzione automatica

激光扫描与波长多光子荧光显微镜的先进光学技术组合的实施是为了捕获高分辨率的、 三维的、 实时成像中甘油三酯 (sox10:EGFP) 和甘油三酯 (foxd3:GFP) 斑马鱼胚胎神经嵴迁移。

Tags

Multi-photon Time Lapse ImagingZebrafish EmbryosNeural Crest Cell MigrationBiologia dello sviluppoCell Migration VisualizationConfocal MicroscopyMulti-photon LaserEmbryo CollectionTransgenic EmbryosGFPPTULowMelt AgaroseOpen Bath Chamber

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