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测定蓝细菌中的糖原含量
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Biochimica
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JoVE Journal Biochimica
Determination of the Glycogen Content in Cyanobacteria
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测定蓝细菌中的糖原含量

DOI:
10.3791/56068-v

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07:04 min

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July 17, 2017

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, ,
1Carlsberg Research Laboratory, 2Department of Biology,University of Copenhagen, 3Copenhagen Plant Science Center, Department of Plant and Environmental Sciences,University of Copenhagen

Capitoli

  • 00:05Titolo
  • 01:01Lysis of Cyanobacterial Cells
  • 02:41Glycogen Precipitation, Enzymatic Hydrolysis, and Glycogen Determination
  • 04:30Determination of Total Glucose Content by the Glucose Oxidase-peroxidase (GOD-POD) Reagent
  • 05:30Results: Measurement of Glycogen Contents in Synechocystis sp.
  • 06:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们提出了一个可靠和容易的测定来测量蓝细菌细胞中的糖原含量。该过程需要沉淀，可选择解聚和葡萄糖残留的检测。该方法适用于野生型和遗传工程菌株，可促进蓝细菌的代谢工程。

Tags

CyanobacteriaGlycogen ContentEnzyme-based AssayMicroorganismsCell LysisProtein ConcentrationChlorophyll A ExtractionEnzyme HydrolysisSpectrophotometric Analysis

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