Here, we present a reliable and easy assay to measure the glycogen content in cyanobacterial cells. The procedure entails precipitation, selectable depolymerization, and the detection of glucose residues. This method is suitable for both wildtype and genetically engineered strains and can facilitate the metabolic engineering of cyanobacteria.
De Porcellinis, A., Frigaard, N., Sakuragi, Y. Determination of the Glycogen Content in Cyanobacteria. J. Vis. Exp. (125), e56068, doi:10.3791/56068 (2017).