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基于磁珠复合法的撕裂细胞因子分析
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
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基于磁珠复合法的撕裂细胞因子分析

11,638 Views

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09:22 min

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October 13, 2017

DOI:

10.3791/55993-v

DOI:
10.3791/55993-v

•

09:22 min
October 13, 2017

•

10 Views
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1National Healthcare Group Eye Institute,Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), 3Institute of Molecular and Cell Biology,A*STAR, 4GROW Research Laboratory,Narayana Nethralaya Foundation, 5Vimta Labs Limited

Summary

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泪膜细胞因子的分析有助于研究各种眼部疾病。基于微珠的复合检测方法简单、灵敏, 能够对小体积样品中的多个目标进行测试。在这里, 我们描述了一个协议的撕裂膜细胞因子分析一个使用珠基复用法。

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Balne, P. K., AU, V. B., Tong, L., Ghosh, A., Agrawal, M., Connolly, J., Agrawal, R. Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles. J. Vis. Exp. (128), e55993, doi:10.3791/55993 (2017).

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