Rivista
/
Neuroscienze
/
Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

DOI:
10.3791/55696-v

•

09:44 min

•

June 22, 2017

•

, , ,
1Division of Radiation Health,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 3Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Capitoli

  • 00:05Titolo
  • 00:55Golgi Staining and Tissue Preparation
  • 02:37Tissue Sectioning
  • 04:33Post-staining Procedures
  • 05:42Mounting, Cleaning, and Covering
  • 07:19Results: Hippocampal Dendritic Complexity in Aged Mice
  • 08:49Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we present a Golgi-Cox protocol in extensive detail. This reliable tissue stain method allows for a high-quality assessment of the cytoarchitecture in the hippocampus, and throughout the entire brain, with minimal troubleshooting.

Tags

Keywords: Golgi-Cox MethodDendritic ComplexityAged MiceNeuroscienzeNeuronal StructureGolgi StainingMercuric ChloridePost-impregnation BufferVibratomePBS

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/55696?article_type=v

Assessment of Motor Balance and Coordination in Mice using the Balance Beam
check_url/it/55696?article_type=v

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
check_url/it/55696?article_type=v

Isolation and Culture of Hippocampal Neurons from Prenatal Mice
check_url/it/55696?article_type=v

Assaying DNA Damage in Hippocampal Neurons Using the Comet Assay
check_url/it/55696?article_type=v

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion
check_url/it/55696?article_type=v

Visualizing the Effects of a Positive Early Experience, Tactile Stimulation, on Dendritic Morphology and Synaptic Connectivity with Golgi-Cox Staining
check_url/it/55696?article_type=v

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
check_url/it/55696?article_type=v

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy
check_url/it/55696?article_type=v

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice
check_url/it/55696?article_type=v

Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons

Read Article