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Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method
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神经科学
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JoVE 杂志 神经科学
Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

Assessment of Hippocampal Dendritic Complexity in Aged Mice Using the Golgi-Cox Method

DOI:
10.3791/55696-v

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09:44 min

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June 22, 2017

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, , ,
1Division of Radiation Health,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 3Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Chapters

  • 00:05Title
  • 00:55Golgi Staining and Tissue Preparation
  • 02:37Tissue Sectioning
  • 04:33Post-staining Procedures
  • 05:42Mounting, Cleaning, and Covering
  • 07:19Results: Hippocampal Dendritic Complexity in Aged Mice
  • 08:49Conclusion

Summary

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Here we present a Golgi-Cox protocol in extensive detail. This reliable tissue stain method allows for a high-quality assessment of the cytoarchitecture in the hippocampus, and throughout the entire brain, with minimal troubleshooting.

Tags

Keywords: Golgi-Cox MethodDendritic ComplexityAged Mice神经科学Neuronal StructureGolgi StainingMercuric ChloridePost-impregnation BufferVibratomePBS

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