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LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics
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JoVE Journal Biochimica
LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics

LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics

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08:01 min

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April 09, 2017

DOI:

10.3791/55626-v

DOI:
10.3791/55626-v

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08:01 min
April 09, 2017

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1Division of Structural Biology and Biochemistry, School of Biological Sciences,Nanyang Technological University

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LERLIC-MS/MSGlutamine DeamidationAsparagine DeamidationShotgun ProteomicsPeptide SeparationAnion Exchange ChromatographyProtein QuantificationDithiothreitolIodoacetamide

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Gallart-Palau, X., Serra, A., Sze, S. K. LERLIC-MS/MS for In-depth Characterization and Quantification of Glutamine and Asparagine Deamidation in Shotgun Proteomics. J. Vis. Exp. (122), e55626, doi:10.3791/55626 (2017).

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