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哺乳动物细胞细菌挑战后应激颗粒形成的免疫荧光分析
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
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哺乳动物细胞细菌挑战后应激颗粒形成的免疫荧光分析

9,955 Views

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11:37 min

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July 03, 2017

DOI:

10.3791/55536-v

DOI:
10.3791/55536-v

•

11:37 min
July 03, 2017

•

5 Views
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1Unité de Pathogénie Microbienne Moléculaire, INSERM U786,Institut Pasteur, 2Microbiologie et Maladies Infectieuses,Collège de France, 3Laboratory of Intestinal Immunity, Institut Imagine-INSERM UMR 1163,Université Paris Descartes-Sorbonne Paris Cité

Tags

ImmunofluorescenceStress GranuleBacterial ChallengeMammalian CellsCellular MicrobiologyPathogenStress Granule PathwayImage AnalysisS.flexneri M90TTryptic Soy AgarTryptic Soy BrothVirulence Gene ExpressionHeLa CellsInfection MediumGentamicin

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Vonaesch, P., Sansonetti, P. J., Schnupf, P. Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells. J. Vis. Exp. (125), e55536, doi:10.3791/55536 (2017).

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