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Immunology and Infection

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JoVE Journal Immunology and Infection
The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections
 

The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

Article DOI: 10.3791/55532-v • 06:57 min • May 8th, 2017
May 8th, 2017 •
1, 2, 2, 2, 2, 1, 1
1Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 2Department of Mechanical and Aerospace Engineering, University of California, San Diego

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The mucus plugged airways of cystic fibrosis (CF) patients are an ideal environment for microbial pathogens to thrive. The manuscript describes a novel method for studying the CF lung microbiome in an environment that mimics where they cause disease and how alterations of chemical conditions can drive microbial dynamics.

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WinCF Model Inexpensive Tractable Microcosm Mucus Plugged Bronchiole Study Microbiology Lung Infections Pathogens Airway Microbiology Cystic Fibrosis Lung Experimental Conditions Microbial Responses Artificial Sputum Medium Potassium Chloride Stock Solution Sodium Chloride Stock Solution Egg Yolk Emulsion DNA Stock Solution Ferritin Stock Solution Essential Amino Acid Solution Non-essential Amino Acid Solution Sterile Water Contamination Incubated Capillary Tubes
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